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Methylthioninium Chloride BP Methylene Blue USP Manufacturers

MSDS of Methylthioninium Chloride Methylene Blue USP Manufacturers

Methylene Blue
Technical & Pure Crystals, Methylthioninium Chloride
Also IP BP Ph. Eur. USP ACS AR LR FCC Food Grades

Methylene Blue

Specifications of Methylene Blue are as follows:
Chemical Formula: C16H18N3CIS
Molecular Weight: 319.85 gm / mole
Chemical Name: 3,7-bis(Dimethylamino)-phenazathionium chloride Tetramethylthionine chloride
CAS Registration: 61-73-4
C.I No.: 52015
Appearance: Dark Green Crystalline Powder
Assay (as is basis): 80 % - 85%
Assay (on dry basis): 98% -101%
Loss on drying: 10% -20%
Solubility: Clear Blue Solution
Ash content: 1% Max
Residual Solvent: Nil
Aluminium: 100 ppm Max
Cadmium: 1ppm Max
Chromium: 10ppm Max
Copper: 50ppm Max
Tin: 10 ppm Max
Iron: 100ppm Max
Manganese: 10ppm Max
Mercury: Nil
Molybdenum: 10ppm Max

Methylene Blue USP

Methylene Blue
C16H18ClN3S·3H2O 373.90
Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride, trihydrate.
C.I. Basic Blue 9 trihydrate [7220-79-3]
Anhydrous 319.86 [61-73-4]

Methylene Blue contains not less than 98.0 percent and not more than 103.0 percent of C16H18ClN3S, calculated on the dried basis.
Identification: Infrared Absorption 197K .

Loss on drying— Dry it at 75 and at a pressure not exceeding 5 mm of mercury for 4 hours: it loses between 8.0% and 18.0% of its weight.
Residue on ignition: not more than 1.2%.
Arsenic— Prepare the Test Preparation by mixing 0.375 g with 10 mL of water in the arsine generator flask. Add 15 mL of nitric acid and 5 mL of perchloric acid, mix, and heat cautiously to the production of strong fumes of perchloric acid. Cool, wash down the sides of the flask with water, and again heat to strong fumes. Again cool, wash down the sides of the flask, and heat to fumes. Cool, dilute with water to 52 mL, and add 3 mL of hydrochloric acid: the resulting solution meets the requirements of the test, the addition of 20 mL of 7 N sulfuric acid specified for Procedure being omitted. The limit is 8 ppm.
Copper or zinc— Ignite 1.0 g in a porcelain crucible, using as low a temperature as practicable, until all of the carbon is oxidized. Cool the residue, add 15 mL of 2 N nitric acid, and boil for 5 minutes. Filter the cooled solution, and wash any residue with 10 mL of water. To the combined filtrate and washing add an excess of 6 N ammonium hydroxide, and filter the solution into a 50 mL volumetric flask. Wash the precipitate with small portions of water, adding the washings to the filtrate, dilute the solution with water to volume, and mix. To 25 mL of the solution add 10 mL of hydrogen sulfide TS: no turbidity is produced within 5 minutes (absence of zinc). Any dark color produced does not exceed that of a control prepared by boiling a quantity of cupric sulfate, equivalent to 200 @g of copper, with 15 mL of 2 N nitric acid for 5 minutes and by treating this solution as directed above, beginning with “Filter the cooled solution” (0.02% of copper).
Chromatographic purity— Quantitatively dissolve an accurately weighed quantity of Methylene Blue in methanol to obtain a Test solution containing 1.0 mg per mL Dissolve a suitable quantity of USP Methylene Blue RS in methanol to obtain a Standard solution having a concentration of 100 @g per mL. Quantitatively dilute a portion of this solution with methanol to obtain a Diluted standard solution having a concentration of 10 @g per mL. Apply 5 @L each of the Test solution, the Standard solution, and the Diluted standard solution to a suitable thin-layer chromatographic plate coated with a 0.25-mm layer of octadecylsilanized chromatographic silica gel. Allow the spots to dry, and develop the chromatogram in a chromatographic chamber with a solvent system consisting of a mixture of the upper layer separated from a well-shaken mixture of water, n-butanol, and glacial acetic acid (100:80:20), until the solvent front has moved about three-fourths of the length of the plate. Remove the plate from the chamber, allow the solvent to evaporate, and visually locate the spots on the plate: the RF value of the principal spot in the chromatogram from the Test solution corresponds to that from the Standard solution, and other spots, if present in the chromatogram from the Test solution, consist of a secondary spot that does not exceed in size or intensity, the principal spot obtained from the Standard solution (10%), and not more than two additional spots, neither of which exceeds in size or intensity the principal spot from the Diluted standard solution (1%)Assay— Transfer about 100 mg of Methylene Blue, accurately weighed, to a 250-mL volumetric flask, dissolve in and dilute with diluted alcohol to volume, and mix. Transfer 5.0 mL of this solution to a 100-mL volumetric flask, dilute with diluted alcohol to volume, and mix. Transfer 5.0 mL of this solution to a 50-mL volumetric flask, dilute with diluted alcohol to volume, and mix. This solution contains about 2 @g per mL. Dissolve an accurately weighed quantity of USP Methylene Blue RS in diluted alcohol, and dilute quantitatively and stepwise with diluted alcohol to obtain a Standard solution having a known concentration of about 2 @g per mL. Concomitantly determine the absorbance of both solutions in 1-cm cells at the wavelength of maximum absorbance at about 663 nm, with a suitable spectrophotometer, using diluted alcohol as the blank. Calculate the quantity, in mg, of C16H18ClN3S in the Methylene Blue taken by the formula:

50C(AU / AS)

in which C is the concentration, in @g per mL, of anhydrous methylene blue in the Standard solution; and AU and AS are the absorbance of the solution of Methylene Blue and the Standard solution, respectively. 

Methylthioninium Chloride BP or Methylthioninium Chloride Ph. Eur is also offered.

IUPAC name is 3,7-Bis(dimethylamino)phenothiazin-5-ylium chloride or 3,7-bis(Dimethylamino)-phenothiazin-5-ium chloride

Methylene Blue USP Manufacturers
Methylthioninium Chloride Manufacturers

Muby Chem Ltd.
Toll manufacturing & Warehouse at Houston Beasley & Midland, Texas, USA
USA, Canada, Mexico Customers may e-mail: usa@mubygroup.com
Call 1-844-682-9243

Europe Africa Asia India Mumbai e-mail: info@mubygroup.com
Call +91-22-23774610

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 26 June, 2015. Copyright and Usual Disclaimer is Applicable.

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Methylene Blue USP Methylthioninium Chloride BP Manufacturers

If you feed a dog with your hand, it will never bite you. That is the principal difference between a dog and a man.

Methylene Blue is also called ( CAS No. 548-62-9; 603-47-4 )
Basic blue 9 (C.I. 52015)
C.I. 52015
Methylthionine chloride
Solvent blue 8 (C.I. 52015)
Swiss blue
Tetramethylthionine chloride
Urolene blue